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Calcium Signals and Calcium Wave Pacemakers (PM) in Ascidian Eggs
570 Ko

Meiotic Calcium Waves Pacemaker PM2 in action: Between meiosis I and meiosis II, 6 to 12 calcium waves are generated by pacemaker PM2 situated in the vegetal contraction pole . These repetitive calcium waves traverse the egg and produce cycles of contractions/relaxations.
Dumollard & Sardet, C. (2001), J. Cell Science, 114, 2471-2481.
Roegiers, F. et al. (1999). Development, 126, 3101-3117
McDougall, A. & Sardet, C. (1995), Current Biology. 5, 318-328.

1.3Mo

Mixing natural and artificial Calcium Waves Pacemakers: Counterwaves can be emitted by a third calcium wave pacemaker (PM3) located in the animal pole cortex when uncaging IP3 between natural waves emitted by calcium wave pacemaker PM2
Dumollard & Sardet, C. (2001), J. Cell Science, 114, 2471-2481.
1.6 Mo

The first Calcium Wave Pacemaker PM1: An activating wave of calcium starts from the sperm entry site and travels vegetally as the egg cortex contracts in response to the activating wave of calcium.Pacemaker PM1 moves vegetally in the cotex with the moving sperm aster.
Dumollard & Sardet, C. (2001), J. Cell Science, 114, 2471-2481.
McDougall, A. & Sardet, C. (1995), Current Biology. 5, 318-328.

500 Ko
 Endoplasmic Reticulum Domains in Ascidian Eggs: Distribution of ER-rich domains in an unfertilized ascidian egg injected with DiIC(16)3. Confocal sections through an egg positioned with the animal pole on top.The vegetal mitochondria-rich myoplasm is ER-poor and traverses by ER-rich corridors
Dumollard & Sardet, C. (2001), J. Cell Science, 114, 2471-2481
700 Ko
 ER-rich Domains are also Cytosol-rich domains: Egg injected with CG dextran and imaged simultaneously with DiIC(16)3 show that ER-rich domains are also Cytosol-rich(see preceeding sequence)
Dumollard & Sardet, C. (2001), J. Cell Science, 114, 2471-2481.