From fertilization to first
cleavage. Fertilization triggers a contraction (the first major
phase of Ooplasmic Segregation) and the emission of 2 polar bodies in 5
and 25 minutes, the migration and fusion of male and female pronuclei and
large rotational movements of the posterior cytoplasm with respect to the
cortex before cleavagewhich takes place 50 minutes after Fertilization
.Sequence recorded with Shinya Inoue.
Sardet, C., et al. (1989).
Development 105, 237-249
Fertilization, contraction and concentration
of the Mitochondria-rich Myoplasm Domain Vegetally during the First Major
Phase of Ooplasmic Segregation; Two eggs with
their mitochondria labelled with DiO(C2)3 undergo an Actomyosin-driven
contraction cortical contraction triggered by the Fertilization Calcium
Wave and lasting 3-5 minutes. A Vegetal/contraction pole is formed which
corresponds to the future Dorsal pole of the Ascidian Embryo(Phallusia
mammillata).
Sardet, C., et al. (1989). Development 105,
237-249
Roegiers F.,et al. (1995) Development, 121,
3457-3466