5
A-- Collecting biological material
B-- Fertilization and culture of embryos
C-- Making chamber for time lapse observation of development
D-- In vivo labelling of ascidian eggs and embryos
E-- Fixations for light and electron microscopy
F-- Labelling cytoskeletal elements of fixed ascidian eggs and embryos
G--Treatment of eggs and embryos with cytoskeletal inhibitors
H-- Micromanipulations of eggs and zygotes
I-- Isolation and Characterization of cortices isolated from ascidian eggs and embryos
