Fertilization and Development of Ctenophores


Ctenophores commonly called " Comb Jellies " or " Moon Jellies " are gelatinous macroplanctonic marine animals. Ctenophores move within currents using 8 rows of irridescent comb rows called " ctenes ".
Most of the 90 species of ctenophores are hermaphrodites which produce eggs and sperm along 8 meridional canals situated beneath each comb row. Ctenophores develop directly and are characterized by an apical sense organ (statocyst) at the aboral pole, a large extensible mouth at the oral pole, and 3 types of tissues (triploblasts).
Beroe are large carnivorous ctenophores that may feed on other ctenophores like Pleurobrachia which are commonly called " cat’s eyes " or " sea gooseberry ". Different species of Beroe and other ctenophores can be collected in the bay of Villefranche sur Mer from February through May. We have used Beroe ovata, which produces very large (1mm diameter) transparent eggs, to study fertilization, pronuclear migration, surface contraction waves and the acquisition of the unique oral-aboral embryonic axis.

Fertilization and polyspermy in Beroe ovata
 
Female pronucleus migrations and polyspermy female pronucleus can choose amongst several male pronuclei ! Female pronucleus migrations and polyspermy female pronucleus can choose amongst several male pronuclei !

Setting up the oral aboral axis in ctenophores (a link to Evelyn Houliston's page)

Collecting Beroe ovata in the Bay of Villefranche