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Living
unfertilized eggs of the sea urchin Paracentrotus lividus In
1901 Boveri used the subequatorial band of pigmented vesicles as a
cortical markers of animal-vegetal polarity to link clivage patterns
and developmental axis formation in sea urchin eggs.
(bright field optics, blue filter) |
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An egg (top left) and an isolated
cortex (bottom right) from the unfertilized egg of the sea urchin Strongylocentrotus
purpuratus side by side. Endoplasmic Reticulum network (red), and cortical granules
(ocre) remain attached to the plasma membrane covered by microvilli. (colorized
Electron micrograph; thin section)
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Cortices isolated from the sea urchin Paracentrotus
lividus after attachment to a polylysine-coated surface(left) or after
homgeneisation (right) The subequatorial band of pigmented vesicles is retained in
cortices. The cortex in the lower right corner in the left image also retained
cortical granules and pigmented granules.
(dark field optics, blue filter, on left) |
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Oblique tangential section through a cortex
isolated from the unfertilized egg of Strongylocentrotus purpuratus
. The cortical Endoplasmic
Reticulum network (red) surrounds cortical granules (ocre). Microvilli and
plasma membrane are on top. (colorized Electron Micrograph; thin section)
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External face of the egg plasma membrane from
the unfertilized sea urchin Paracentrotus lividus. The membrane is studded with
short micropapillae. A lawn of tightly packed cortical granules (ocre) is
situated beneath the surface.
(colorized Electron Micrograph, freeze fracture replica) |
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Isolated cortex of the unfertilized egg of the
sea urchin Strongylocentrotus purpuratus. The vesicles on the left are cortical
granules attached to the plasma membrane (DIC optics). On the right the
Endoplasmic Reticulum network (red EpiFluorescence Optics) is labelled with
the lipophilic dye DiIC16(3)in the same cortex .
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