Images of the Egg Cortex

Sea urchin egg
and isolated cortices



Living unfertilized eggs of the sea urchin Paracentrotus lividus In 1901 Boveri used the subequatorial band of pigmented vesicles as a cortical markers of animal-vegetal polarity to link clivage patterns and developmental axis formation in sea urchin eggs.
(bright field optics, blue filter)


An egg (top left) and an isolated cortex (bottom right) from the unfertilized egg of the sea urchin Strongylocentrotus purpuratus side by side. Endoplasmic Reticulum network (red), and cortical granules (ocre) remain attached to the plasma membrane covered by microvilli. (colorized Electron micrograph; thin section)

Cortices isolated from the sea urchin Paracentrotus lividus after attachment to a polylysine-coated surface(left) or after homgeneisation (right) The subequatorial band of pigmented vesicles is retained in cortices. The cortex in the lower right corner in the left image also retained cortical granules and pigmented granules.
(dark field optics, blue filter, on left)




Oblique tangential section through a cortex isolated from the unfertilized egg of Strongylocentrotus purpuratus . The cortical Endoplasmic Reticulum network (red) surrounds cortical granules (ocre). Microvilli and plasma membrane are on top. (colorized Electron Micrograph; thin section)



External face of the egg plasma membrane from the unfertilized sea urchin Paracentrotus lividus. The membrane is studded with short micropapillae. A lawn of tightly packed cortical granules (ocre) is situated beneath the surface.
(colorized Electron Micrograph, freeze fracture replica)







Isolated cortex of the unfertilized egg of the sea urchin Strongylocentrotus purpuratus. The vesicles on the left are cortical granules attached to the plasma membrane (DIC optics). On the right the Endoplasmic Reticulum network (red EpiFluorescence Optics) is labelled with the lipophilic dye DiIC16(3)in the same cortex .