Ovulation in an
isolated gonad (1.1
MB) - Colour Film by Christian Sardet and Dany Carré.
Ovulation
(0.9MB) in an isolated gonad , trigered by light after a 8 hour
dark period (avi
version: 5.7MB).
This film spans 2 hours with 1 image taken every 2 minutes.
The same gonad was filmed over an 18 hour period the previous day to
follow oocyte
growth (5.6 MB)
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Ooctye
Maturation in an isolated oocyte triggered by incubation in
in Br-cAMP (11 MB). The film spans the 2h maturation period.
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The first division (1.3 MB)
of the fertilised egg (avi version : 540 Ko). Division in
unipolar, starting from the animal pole and cutting down to the vegetal
pole. Unipolar divisions occur in the early embryo because nuclei and
spindles are confined to a thin peripheral layer of cytoplasm, and so
stimulate cytokinesis locally rather than in a ring.
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Cleavage divisions
(3.9MB). 1 cell stage to late blastula stage
Early blastula stage embryos are irregular in
shape -->
but most commonly they form an asymmetric "peanut" (5MB) or sometimes a "twisted heart" (5MB), with a main cleft
corresponding to the original animal pole of the egg,
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Gastrulation
by unipolar ingression
(3.5 MB). Images taken every 10 minutes, about 12h total. It's hard to
keep the embryo still from the beginning of gastrulation onwards,
because it grows cilia and starts to swim!. In this film we tried
embedding the embryo in agar.....
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Metamorphosis
Formation of primary polyps from a 3 day old planula larvae.
Film 1
(2.2 MB). Covers an 8 hour period with images taken every 5
minutes, starting about 5h after stimulation (3h incubation in 58mM
CsCl)
Film 2
(3 MB). Covers a 22 hour period, with images taken every 6 minutes,
starting just after stimulation (3h treatment with 58mM CsCl)
Film 3
(0.3 MB). Spanning a 4 hour period at the end of polyp
formation, images taken every 6 minutes, starting about 20h after
stimulation by CsCl.
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Feeding and
medusa release (3.1 MB) (avi
version 4.2 MB) images taken every 6 minutes, about 20h total).
The gonozoids are swallowing artemia!
Release of medusae
from gonozooids (0.2MB) images taken every 30 seconds for 22 minutes (avi version: 3.0 MB).
Another film of medusa budding seen
from the side (3.3 MB)
and a shorter, slowed-down
version (0.5 MB)
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Adult
Clytia Swimming : (0.8
MB)
- Colour Film by Christian Sardet and Dany Carré.
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Condensed chromosomes at the
onset of mitosis at around the 32 cell stage
Z series (57 Ko)
3D projection (119 Ko)
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