- UNIVERSITE P. ET M. CURIE - CNRS -

french_flag

image
Dévelopmental     Biology     Research     -  UMR7009 BioDev  -   CNRS
Home > ClytiaPhotosFilms

Clytia hemisphaerica   -    Gallery

filet




back to....

  Clytia home page

  Life cycle &culture

  Research

  Bibliography

  Cnidarian links

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 








      

 Egg: 240 µm diameter
      (animal pole at top)        

 gastrula
( future oral pole at top)

2 day planula
  ( oral pole at top)

 

 

 

      

polyp forming by metamorphosis

 gastrozoid polyp

gonozoid polyp

 

 

 

       

 newly released baby medusa

 adult medusa  (1-2 cm in diameter)

female gonad


                                                                                                             (Photos : Tsuyoshi Momose)

 

FILMS

Ovulation in an isolated gonad (1.1 MB) - Colour Film by Christian Sardet and Dany Carré.

Ovulation (0.9MB) in an isolated gonad , trigered by light after a 8 hour dark period  (avi version: 5.7MB).
This film spans 2 hours  with 1 image taken every 2 minutes.
The same gonad was filmed over an 18 hour period the previous day to follow oocyte growth (5.6 MB)

Ooctye Maturation in an isolated oocyte triggered by incubation in  in Br-cAMP (11 MB). The film spans the 2h maturation period.

The first division (1.3 MB) of the fertilised egg (avi version : 540 Ko). Division in unipolar, starting from the animal pole and cutting down to the vegetal pole. Unipolar divisions occur in the early embryo because nuclei and spindles are confined to a thin peripheral layer of cytoplasm, and so stimulate cytokinesis locally rather than in a ring.

Cleavage divisions (3.9MB). 1 cell stage to late blastula stage

- about 8 h total  (avi version: 5.1 MB).


Early blastula stage embryos are irregular in shape  -->

but most commonly they form an asymmetric "peanut" (5MB) or sometimes a "twisted heart" (5MB), with a main cleft corresponding to the original animal pole of the egg,

Gastrulation by unipolar ingression (3.5 MB). Images taken every 10 minutes, about 12h total. It's hard to keep the embryo still from the beginning of gastrulation onwards, because it grows cilia and starts to swim!. In this film we tried embedding the embryo in agar.....

Metamorphosis
 Formation of primary polyps from a 3 day old planula larvae.
Film 1 (2.2 MB).  Covers an 8 hour period with images taken every 5 minutes, starting about 5h after stimulation (3h incubation in 58mM CsCl)
Film 2 (3 MB). Covers a 22 hour period, with images taken every 6 minutes, starting just  after stimulation (3h treatment with 58mM CsCl)
Film 3 (0.3 MB).  Spanning a 4 hour period at the end of polyp formation,  images taken every 6 minutes, starting about 20h after stimulation by CsCl.

Stolon Growth (6 MB)  (uncompressed version: 16 MB)               
 
images taken every 10 minutes , about 40h total.                    

Feeding and medusa release (3.1 MB) (avi version 4.2 MB)  images taken every 6 minutes, about 20h total). The gonozoids are swallowing artemia!

Release of medusae from gonozooids (0.2MB) images taken every 30 seconds for 22 minutes (avi version: 3.0 MB).

Another film of medusa budding seen from the side (3.3 MB) and a shorter,  slowed-down version (0.5 MB)

                                                                                                                     

Adult Clytia Swimming : (0.8 MB)
- Colour Film by Christian Sardet and Dany Carré.

Condensed chromosomes at the onset of mitosis at around the 32 cell stage

Z series  (57 Ko)

3D projection  (119 Ko)



                                         (Film production : Evelyn Houliston, Aldine Amiel, Christian Rouvière)




UMR7009 CNRS/UPMC Biologie du Développement
Observatoire Océanologique de Villefranche-sur-Mer
Quai de la Darse, 06234 Villefranche-sur-Mer Cedex France
Tél : +33 4 93 76 37 70, Fax : +33 4 93 76 37 92
© Copyright CNRS BioDev